Purospher® RP-18
Accelerate and simplify method development for basic compounds
Purospher® RP-18 is designed for peak-tailing free chromatography of strongly basic compounds with simple, neutral mobile phases. Thus, method development time and consequently analyses costs are reduced. Additionally, Purospher® RP-18 allows the separation of hydrophilic compounds using up to 100 % aqueous eluents.
The key for understanding the properties of Purospher® RP-18 lies in the chemistry of the base, high purity silica with virtually no metal contaminants present. In addition, multi-step chemical modification and deactivation by polymeric coating and amino shielding of the surface eliminate unpredictable interactions from residual silanols. This results in symmetric peak shapes of basic and chelating analytes without the addition of any modifiers to the mobile phase.
Due to the amino endcapping step, Purospher® RP-18 is not suitable for the separation of acidic compounds.
The high chemical stability of Purospher® RP-18 permits the usage of mobile phase conditions at pH 8 for a long period of time without decline of performance.
| Key performance benefits: |
|---|
|
| Specification of Purospher® RP-18 | |
|---|---|
| Sorbent characteristics | High purity C18-modified silica with deactivated surface. Due to the amino shielding not suitable for separation of acidic compounds! |
| Particle shape | spherical |
| Particle size | 5 μm |
| Pore size | 90 Å (9 nm) |
| Pore volume | 1.05 mL/g |
| Spec. surface area | 480 m²/g |
| Carbon load | 17 % C |
| pH range | pH 2 - 8 |
| Efficiency | 80.000 N/m |
| Shipping eluent | Acetonitrile/Water |
| Separation of Nucleic Acids on Purospher® RP-18 | |||
|---|---|---|---|
| Column | LiChroCART® 250-4 Purospher® RP-18, 5 µm |  |
|
| Eluent | 1 % Acetic acid | ||
| Flow rate | 1.0 mL/min | ||
| Detection | UV 214 nm | ||
| Temperature | 22°C | ||
| Inj. volume | 1 µL | ||
| Sample | 1. Cytosine 2. Adenine 3. Guanine 4. Uracil 5. 5-Fluorouracil 6. Hypoxanthine 7. Xanthine 8. Thymine |
5.5 µg/mL 10 µg/mL 14 µg/mL 10 µg/mL 24 µg/mL 11 µg/mL 14 µg/mL 14 µg/mL |
|
